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Received — 10 September 2026 ⏭ Nanotechnology

Molecular Therapy Advances
Automated Zonal-Rotor CsCl Ultracentrifugation with ÄKTA-Based Fractionation Removes Empty and Intermediate AAV Capsids Kosuke Horita · Ryuki Miyauchi · Hideaki Yamamoto · Yasuhiro Yamazaki · Kentaro Nagatomo · Takahide Aburatani · Shuji Takeda · Satoshi Baba
Automated ÄKTA-connected zonal-rotor CsCl ultracentrifugation enables high-resolution AAV polishing at 1.65-L scale. The workflow enriched full capsids and resolved empty and intermediate populations in AAV8-X and AAV9-Y. The AAV8-X intermediate fraction retained about a half of qPCR signal of equimolar full capsid but produced minimal mRNA expression potency.
1 September 2026 at 08:00

Automated Zonal-Rotor CsCl Ultracentrifugation with ÄKTA-Based Fractionation Removes Empty and Intermediate AAV Capsids

By: Kosuke Horita · Ryuki Miyauchi · Hideaki Yamamoto · Yasuhiro Yamazaki · Kentaro Nagatomo · Takahide Aburatani · Shuji Takeda · Satoshi Baba

1 September 2026 at 08:00

Automated ÄKTA-connected zonal-rotor CsCl ultracentrifugation enables high-resolution AAV polishing at 1.65-L scale. The workflow enriched full capsids and resolved empty and intermediate populations in AAV8-X and AAV9-Y. The AAV8-X intermediate fraction retained about a half of qPCR signal of equimolar full capsid but produced minimal mRNA expression potency.