By selectively targeting peripheral 5-HT2AR without inducing psychedelic effects, a non-brain-penetrant agonist boosts antitumor CD8+ T cell immunity and improves immunotherapy responses in preclinical models of colorectal cancer.

A mitochondrial transplantation approach rescues mitochondrial deficiency and prevents mitochondrial DNA depletion syndrome, Leigh syndrome, and Parkinson’s disease in cellular and mouse models.

The RNA sequence of HIV-1 TAR was systematically altered to change its propensity to adopt a functional secondary structure in the ensemble, measured using 1H CEST NMR. These minor sequence changes shifted the active-state propensity by ∼500-fold, quantitatively predicting changes in protein binding and cellular transactivation. These propensities could be inferred from secondary-structure prediction algorithms and incorporated into a thermodynamic framework to quantitatively predict how sequence changes alter protein-binding affinity and RNA cellular activity.