Nature Biotechnology, Published online: 01 April 2026; doi:10.1038/s41587-026-03051-1ERAST speeds up homology search and provides a vector database for 1 billion biological sequences.

Nature Biotechnology, Published online: 31 March 2026; doi:10.1038/s41587-026-03068-6Simultaneous profiling of adenylated and non-adenylated RNAs reveals regulatory programs across diverse cell types.

Nature Biotechnology, Published online: 31 March 2026; doi:10.1038/s41587-026-03083-7Single-molecule 4Pi microscopy is simplified and made accessible by using a single objective.

Nature Biotechnology, Published online: 30 March 2026; doi:10.1038/s41587-026-03060-0Non-transgenic crop improvement via cis-regulatory editing is limited by rare activating alleles and incomplete promoter maps. A massively parallel reporter assay in sorghum protoplasts quantifies more than 30,000 CRISPR-mimicking variants across three photosynthesis genes, finely mapping cis-regulatory modules and pinpointing compact edits that drive upregulated protein production.

Nature Biotechnology, Published online: 30 March 2026; doi:10.1038/s41587-026-03104-5Immune cell engagers (ICEs) have emerged as a promising strategy for cancer immunotherapy, but their efficacy in solid tumors remains limited, partly as a result of the difficulty of coordinating activating and inhibitory signals within the same immune cell. Here, we report a logic-gated cis-targeting strategy that enables ICEs to coordinate activating and inhibitory signals within macrophages, thereby enhancing

Nature Biotechnology, Published online: 27 March 2026; doi:10.1038/s41587-026-03074-8Unpaired data as a first-order challenge in single-cell and spatial proteomics

Nature Biotechnology, Published online: 27 March 2026; doi:10.1038/s41587-026-03046-yVariant mapping of sorghum promoters identifies CRISPR-accessible mutations that upregulate gene expression.

Nature Biotechnology, Published online: 27 March 2026; doi:10.1038/s41587-026-03053-zA long-read metagenome assembly method recovers circular and complete genomes better than existing tools.