This study demonstrates that extrachromosomal DNA (ecDNA) replication induces DNA double-strand breaks and activates the DNA damage response (DDR). The DDR pathways, such as alt-NHEJ, are critical for ecDNA maintenance in tumor cells. Mechanistic insights into ecDNA replication and maintenance unveil a therapeutic approach for treating tumors harboring ecDNA.

Complex disorders require precise strategies for their characterization. AI-based digital phenotypes from biosensors can be used to predict psychiatric disorders and identify GWAS loci.