❌

Normal view

Unraveling the role of cuproptosis in pulmonary fibrosis pathogenesis and prognosis: an integrative single-cell transcriptomics and microarray analysis

13 March 2026 at 18:00

Mol Cell Biochem. 2026 Mar 13. doi: 10.1007/s11010-026-05510-4. Online ahead of print.

ABSTRACT

Pulmonary fibrosis (PF), a progressive interstitial lung disease with elusive pathogenesis, remains a therapeutic challenge. Emerging evidence suggests cuproptosis-a copper-dependent cell death pathway-may play a regulatory role in disease progression. This study aims to elucidate cuproptosis's biological function and establish a prognostic model for PF. Through integrative analysis of single-cell RNA-seq data from bleomycin (BLM)-induced mouse models and bulk RNA-seq data from idiopathic pulmonary fibrosis (IPF) patients, we identified cuproptosis-related genes (CRGs) using LASSO regression and Cox regression. A novel 4-CRG signature (LIAS, LIPT1, ATP7A, PDHB) was constructed to stratify patients into distinct risk groups in the GSE70866 cohort, where high-risk individuals exhibited poorer survival and enhanced extracellular matrix/lipid metabolism activity via GO/KEGG analysis. Experimental validation in BLM-induced mouse models, TGF-Ξ²1-stimulated fibroblast-to-myofibroblast transition assays, and human IPF specimens demonstrated significant downregulation of CRGs through qRT-PCR and immunohistochemical analyses. Functional assays revealed impaired cell viability and elevated cuproptosis markers in fibrotic microenvironments. Our findings establish an inverse correlation between cuproptosis and PF progression, and propose a robust risk-score model for clinical prognosis prediction. This multi-omics approach provides new insights into copper-mediated regulatory mechanisms in fibrogenesis.

PMID:41824199 | DOI:10.1007/s11010-026-05510-4

REVISION:Reflective Intent Mining and Online Reasoning Auxiliary for E-commerce Visual Search System Optimization

arXiv:2510.22739v2 Announce Type: replace-cross Abstract: In Taobao e-commerce visual search, user behavior analysis reveals a large proportion of no-click requests, suggesting diverse and implicit user intents. These intents are expressed in various forms and are difficult to mine and discover, thereby leading to the limited adaptability and lag in platform strategies. This greatly restricts users' ability to express diverse intents and hinders the scalability of the visual search system. This mismatch between user implicit intent expression and system response defines the User-SearchSys Intent Discrepancy. To alleviate the issue, we propose a novel framework REVISION. This framework integrates offline reasoning mining with online decision-making and execution, enabling adaptive strategies to solve implicit user demands. In the offline stage, we construct a periodic pipeline to mine discrepancies from historical no-click requests. Leveraging large models, we analyze implicit intent factors and infer optimal suggestions by jointly reasoning over query and product metadata. These inferred suggestions serve as actionable insights for refining platform strategies. In the online stage, REVISION-R1-3B, trained on the curated offline data, performs holistic analysis over query images and associated historical products to generate optimization plans and adaptively schedule strategies across the search pipeline. Our framework offers a streamlined paradigm for integrating large models with traditional search systems, enabling end-to-end intelligent optimization across information aggregation and user interaction. Experimental results demonstrate that our approach improves the efficiency of implicit intent mining from large-scale search logs and significantly reduces the no-click rate.

Pailitao-VL: Unified Embedding and Reranker for Real-Time Multi-Modal Industrial Search

arXiv:2602.13704v1 Announce Type: cross Abstract: In this work, we presented Pailitao-VL, a comprehensive multi-modal retrieval system engineered for high-precision, real-time industrial search. We here address three critical challenges in the current SOTA solution: insufficient retrieval granularity, vulnerability to environmental noise, and prohibitive efficiency-performance gap. Our primary contribution lies in two fundamental paradigm shifts. First, we transitioned the embedding paradigm from traditional contrastive learning to an absolute ID-recognition task. Through anchoring instances to a globally consistent latent space defined by billions of semantic prototypes, we successfully overcome the stochasticity and granularity bottlenecks inherent in existing embedding solutions. Second, we evolved the generative reranker from isolated pointwise evaluation to the compare-and-calibrate listwise policy. By synergizing chunk-based comparative reasoning with calibrated absolute relevance scoring, the system achieves nuanced discriminative resolution while circumventing the prohibitive latency typically associated with conventional reranking methods. Extensive offline benchmarks and online A/B tests on Alibaba e-commerce platform confirm that Pailitao-VL achieves state-of-the-art performance and delivers substantial business impact. This work demonstrates a robust and scalable path for deploying advanced MLLM-based retrieval architectures in demanding, large-scale production environments.
❌