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Orally Administered Porcine Intestinal Lactobacilli Improve the Respiratory Innate Immune Response Against <em>Streptococcus pneumoniae</em>

Animals (Basel). 2026 Mar 6;16(5):825. doi: 10.3390/ani16050825.

ABSTRACT

BACKGROUND: Respiratory bacterial infections represent a major health challenge in swine production, highlighting the need for novel immunomodulatory strategies that enhance host resistance. In this study, we investigated whether porcine intestinal lactobacilli could modulate the gut-lung axis and improve respiratory innate immunity in a mouse model of Streptococcus pneumoniae infection, as a surrogate of Streptococcus suis pneumonia.

METHODS: Three strains of Ligilactobacillus salivarius (LAFF998, LAFF1071, and LAFF1095) were orally administered to Swiss mice prior to pneumococcal challenge. The resistance to the infection, the lung damage and the respiratory innate immune response were evaluated.

RESULTS: Only strain LAFF998 significantly reduced pulmonary bacterial loads, prevented bacteremia, and attenuated lung injury. This protective effect was associated with selective modulation of respiratory immunity, characterized by reduced neutrophilic inflammation, increased lymphocyte recruitment, and enhanced activation of alveolar macrophages expressing MHC-II. LAFF998 markedly increased the production of IFN-β, IFN-γ, IL-6, IL-10, and IL-27 in the respiratory tract, without inducing excessive inflammatory damage. Ex vivo and in vitro analyses confirmed that alveolar macrophages from LAFF998-treated mice exhibited a primed phenotype with heightened cytokine responses to pneumococcal stimulation. In contrast, strains LAFF1071 and LAFF1095 failed to confer protection or significantly modulate respiratory immune responses.

CONCLUSIONS: These findings demonstrate a strict strain-dependent effect among porcine L. salivarius isolates and identify LAFF998 as a potent immunobiotic capable of enhancing respiratory innate immunity through the gut-lung axis. This work supports further studies of LAFF998 as an immunobiotic strategy for the prevention of respiratory infections in pigs.

PMID:41829035 | PMC:PMC12985233 | DOI:10.3390/ani16050825

NFATC2::NUTM2 Fusion Defines a Novel Primary Pulmonary Epithelial Tumor With a Distinctive Immunophenotype

Am J Surg Pathol. 2026 Jun 1;50(6):695-704. doi: 10.1097/PAS.0000000000002533. Epub 2026 Mar 13.

ABSTRACT

With the application of molecular techniques in pathologic diagnosis, several novel primary pulmonary epithelial tumors have been continuously discovered and classified under the WHO classification of thoracic tumors. Recently, a pulmonary tumor with NFATC2 :: NUTM2B fusion was first documented, but the spectrum of NFATC2::NUTM2 fusion variants and their associated pathologic features remains incompletely characterized. Coincidentally, we also found and described 6 primary pulmonary tumors harboring recurrent NFATC2::NUTM2A/E fusions through integrated genomic analysis. These patients, including 4 females and 2 males, with a median age of 53 years, presented with incidentally detected peripheral lung nodules composed of monotonous epithelioid cells arranged in cords, nests, and trabeculae within a prominent desmoplastic stroma. All tumors exhibited a consistent immunophenotype: CK5/6+/GATA3+/calponin+/EMA+/DOG1 (perinuclear dot-like staining)/p63-. High-throughput chromosome conformation capture (Hi-C) analysis showed the structural variation of NFATC2::NUTM2E in all 6 cases, whereas RNA sequencing detected the fusion transcripts in 5 cases ( NFATC2::NUTM2A , n=2; NFATC2::NUTM2E , n=3). Ultrastructural examination of 1 case suggested epithelial differentiation. All patients remained disease-free after complete resection (median follow-up: 24 mo; range: 9 to 41 mo). These findings define a novel primary pulmonary tumor entity driven by NFATC2::NUTM2 fusions, and characterized by a distinctive immunophenotype, expanding the spectrum of NUTM2 -associated neoplasms. Our study underscores the utility of multiomics approaches for characterizing rare neoplasms and provides a diagnostic framework for this entity.

PMID:41821426 | DOI:10.1097/PAS.0000000000002533

Post-Acute Sequelae of COVID-19 Persist Over 3 Years in Acute Lung Injury/Acute Respiratory Distress Syndrome Survivors But Are Not Associated With Persistent Thromboinflammation or Endothelial Dysfunction

Crit Care Explor. 2026 Mar 12;8(3):e1390. doi: 10.1097/CCE.0000000000001390. eCollection 2026 Mar 1.

ABSTRACT

IMPORTANCE: Inflammation, endothelial dysfunction, and complement activation are associated with COVID-19 acute lung injury (ALI) and acute respiratory distress syndrome (ARDS).

OBJECTIVES: We hypothesized that higher levels of inflammation, endothelial dysfunction, and complement activation implicated in more severe COVID-19 ALI/ARDS are associated with post-acute sequelae of COVID-19 (PASC) phenotypes in the 3 years after hospitalization.

DESIGN, SETTING, AND PARTICIPANTS: A single-center prospective cohort of 150 adult survivors of severe and critical COVID-19 from the first wave of the pandemic with sampling weighted to include 50% survivors of mechanical ventilation.

MAIN OUTCOMES AND MEASURES: Eleven serum biomarkers at hospital discharge, 4 months, 15 months, and 3 years, and symptoms and physical function at 15 months and 3 years. PASC presence was defined using the 12 symptoms and scoring from the Researching COVID to Enhance Recovery (RECOVER) definition. We tested associations of biomarkers with PASC and symptom phenotypes of post-exertional malaise, fatigue, and brain fog while adjusting for age, sex, body mass index, comorbidities, and days since COVID-19 diagnosis.

RESULTS: The mean (sd) age of the cohort was 56 years (13); 67% were Hispanic and 25% were Black. PASC was present in 26% of participants at both 15 months and 3 years. PASC and symptom phenotypes at 15 months and 3 years were consistently associated with higher frailty phenotype category, worse short physical performance battery scores, and shorter 6-minute walk distance. Biomarkers of inflammation, including interleukin-6 and soluble tumor necrosis factor receptor-1, endothelial function, including angiopoietin, and complement, including C2, C4b, and C5, were not associated with PASC or symptom phenotypes in cross-sectional or longitudinal analyses.

CONCLUSIONS AND RELEVANCE: PASC persists for 3 years after acute COVID-19 ALI/ARDS, is associated with frailty, but not associated with persistently higher levels of inflammatory, endothelial, and complement biomarkers implicated in worse short-term outcomes in acute COVID-19, non-COVID-19 ARDS, and sepsis. Future studies should employ multiomics to elucidate potential mechanisms of PASC.

PMID:41824803 | DOI:10.1097/CCE.0000000000001390

Targeting the OXNAD1-PTGS2 axis with resveratrol overcomes ferroptosis Inhibition and reverses 5-FU resistance in gastric cancer

Gastric Cancer. 2026 Mar 13. doi: 10.1007/s10120-026-01718-x. Online ahead of print.

ABSTRACT

BACKGROUND: 5-Fluorouracil (5-FU) remains a cornerstone of first-line chemotherapy for gastric cancer, yet the emergence of resistance severely compromises its clinical efficacy. Although ferroptosis suppression has been recognized as a pivotal mechanism of chemoresistance, the mitochondrial regulatory processes involved remain poorly understood.

METHODS: We integrated clinical specimen analysis, in vitro and in vivo functional assays, multi-omics profiling, and molecular docking to delineate the role of the mitochondrial oxidoreductase OXNAD1 in mediating 5-FU resistance in gastric cancer, and to assess the therapeutic potential of the natural polyphenol resveratrol as a chemosensitizing agent.

RESULTS: OXNAD1 was found to be significantly overexpressed in gastric cancer tissues and cell lines, correlating with unfavorable prognosis and enhanced 5-FU resistance. Mechanistically, OXNAD1 directly bound to and suppressed the ferroptosis driver PTGS2, thereby attenuating lipid peroxidation and mitochondrial damage, ultimately restraining ferroptosis and promoting drug resistance. Notably, resveratrol disrupted the OXNAD1-PTGS2 interaction by directly binding OXNAD1, reinstating ferroptotic activity, markedly enhancing the cytotoxic effect of 5-FU in resistant cells, and potentiating the antitumor efficacy of 5-FU in xenograft models.

CONCLUSION: The OXNAD1-PTGS2 axis constitutes a critical metabolic-cell death cross-regulatory pathway underlying 5-FU resistance in gastric cancer. Targeting this axis with resveratrol provides a promising combinatorial strategy to overcome chemoresistance.

PMID:41824193 | DOI:10.1007/s10120-026-01718-x

Post-Acute Sequelae of COVID-19 Persist Over 3 Years in Acute Lung Injury/Acute Respiratory Distress Syndrome Survivors But Are Not Associated With Persistent Thromboinflammation or Endothelial Dysfunction

Crit Care Explor. 2026 Mar 12;8(3):e1390. doi: 10.1097/CCE.0000000000001390. eCollection 2026 Mar 1.

ABSTRACT

IMPORTANCE: Inflammation, endothelial dysfunction, and complement activation are associated with COVID-19 acute lung injury (ALI) and acute respiratory distress syndrome (ARDS).

OBJECTIVES: We hypothesized that higher levels of inflammation, endothelial dysfunction, and complement activation implicated in more severe COVID-19 ALI/ARDS are associated with post-acute sequelae of COVID-19 (PASC) phenotypes in the 3 years after hospitalization.

DESIGN, SETTING, AND PARTICIPANTS: A single-center prospective cohort of 150 adult survivors of severe and critical COVID-19 from the first wave of the pandemic with sampling weighted to include 50% survivors of mechanical ventilation.

MAIN OUTCOMES AND MEASURES: Eleven serum biomarkers at hospital discharge, 4 months, 15 months, and 3 years, and symptoms and physical function at 15 months and 3 years. PASC presence was defined using the 12 symptoms and scoring from the Researching COVID to Enhance Recovery (RECOVER) definition. We tested associations of biomarkers with PASC and symptom phenotypes of post-exertional malaise, fatigue, and brain fog while adjusting for age, sex, body mass index, comorbidities, and days since COVID-19 diagnosis.

RESULTS: The mean (sd) age of the cohort was 56 years (13); 67% were Hispanic and 25% were Black. PASC was present in 26% of participants at both 15 months and 3 years. PASC and symptom phenotypes at 15 months and 3 years were consistently associated with higher frailty phenotype category, worse short physical performance battery scores, and shorter 6-minute walk distance. Biomarkers of inflammation, including interleukin-6 and soluble tumor necrosis factor receptor-1, endothelial function, including angiopoietin, and complement, including C2, C4b, and C5, were not associated with PASC or symptom phenotypes in cross-sectional or longitudinal analyses.

CONCLUSIONS AND RELEVANCE: PASC persists for 3 years after acute COVID-19 ALI/ARDS, is associated with frailty, but not associated with persistently higher levels of inflammatory, endothelial, and complement biomarkers implicated in worse short-term outcomes in acute COVID-19, non-COVID-19 ARDS, and sepsis. Future studies should employ multiomics to elucidate potential mechanisms of PASC.

PMID:41824803 | PMC:PMC12987405 | DOI:10.1097/CCE.0000000000001390

NFATC2::NUTM2 Fusion Defines a Novel Primary Pulmonary Epithelial Tumor With a Distinctive Immunophenotype

Am J Surg Pathol. 2026 Mar 13. doi: 10.1097/PAS.0000000000002533. Online ahead of print.

ABSTRACT

With the application of molecular techniques in pathologic diagnosis, several novel primary pulmonary epithelial tumors have been continuously discovered and classified under the WHO classification of thoracic tumors. Recently, a pulmonary tumor with NFATC2::NUTM2B fusion was first documented, but the spectrum of NFATC2::NUTM2 fusion variants and their associated pathologic features remains incompletely characterized. Coincidentally, we also found and described 6 primary pulmonary tumors harboring recurrent NFATC2::NUTM2A/E fusions through integrated genomic analysis. These patients, including 4 females and 2 males, with a median age of 53 years, presented with incidentally detected peripheral lung nodules composed of monotonous epithelioid cells arranged in cords, nests, and trabeculae within a prominent desmoplastic stroma. All tumors exhibited a consistent immunophenotype: CK5/6+/GATA3+/calponin+/EMA+/DOG1 (perinuclear dot-like staining)/p63-. High-throughput chromosome conformation capture (Hi-C) analysis showed the structural variation of NFATC2::NUTM2E in all 6 cases, whereas RNA sequencing detected the fusion transcripts in 5 cases (NFATC2::NUTM2A, n=2; NFATC2::NUTM2E, n=3). Ultrastructural examination of 1 case suggested epithelial differentiation. All patients remained disease-free after complete resection (median follow-up: 24 mo; range: 9 to 41 mo). These findings define a novel primary pulmonary tumor entity driven by NFATC2::NUTM2 fusions, and characterized by a distinctive immunophenotype, expanding the spectrum of NUTM2-associated neoplasms. Our study underscores the utility of multiomics approaches for characterizing rare neoplasms and provides a diagnostic framework for this entity.

PMID:41821426 | DOI:10.1097/PAS.0000000000002533

Effects of Digital Health Interventions on Functional and Psychological Outcomes in Older Patients With Hip Fractures: Systematic Review and Meta-Analysis of Randomized Controlled Trials

Background: Hip fractures in older adults increasingly challenge public health, making traditional rehabilitation very challenging. Digital health interventions (DHIs) have emerged as a promising solution for postoperative rehabilitation. However, evidence on DHIs’ effects on functional and psychological outcomes remains insufficient. Objective: This systematic review aimed to comprehensively examine the effects of DHIs on functional and psychological outcomes in older adults with hip fractures. Methods: Following PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines, we searched 9 databases (PubMed, Embase, CENTRAL, APA PsycINFO, Web of Science, PEDro, CNKI, WANFANG, and SinoMed) from inception to November 13, 2025. Included studies enrolled adults aged 60 years and older with hip fractures, delivered DHIs, assessed functional and psychological outcomes, set usual care or no intervention as the control, and had a randomized controlled trial design. Studies were excluded if they enrolled nonhospitalized patients in the emergency department, patients discharged to nonhome settings, or had inaccessible full text or insufficient data. Study quality was evaluated using the Cochrane Risk of Bias tool 2.0 (Cochrane Collaboration), and evidence certainty was assessed using GRADE (Grading of Recommendations, Assessment, Development and Evaluation). The literature screening, data extraction, and quality assessment were independently conducted by 2 researchers, and any disputes were resolved by the third researcher. We performed analysis using R version 4.0.3 (R Foundation for Statistical Computing) with a random-effects model. Results: Of 17,723 studies screened, 13 met the inclusion criteria. DHIs, compared to the control, significantly improved hip function (standardized mean difference [SMD] 0.80, 95% CI 0.33-1.26; 95% prediction interval [PI] –0.24 to 1.83; P=.007) and functional independence (SMD 1.23, 95% CI 0.34-2.11; 95% PI –0.98 to 3.34; P=.02). Despite favorable pooled effects, a wide 95% PI spanning positive or negative values signals substantial heterogeneity. No significant difference was observed in balance function, risk of falling, and quality of life. Only a single available study reported a 70% adherence rate in the DHIs group. Subgroup analyses stratified by intervention duration revealed no significant intersubgroup differences for hip function (χ12=0.1; P=.75) or functional independence (χ12=2.93; P=.09). For hip function, the point estimate favored the 3 months subgroup (SMD 0.89, 95% CI 0.36-1.41; I2=7%; P=.41) over the <3 months subgroup. Conversely, for functional independence, the point estimate favored shorter intervention duration (SMD 0.67, 95% CI 0.12-1.23; I²=0%; P=.72). Conclusions: This review incorporates the latest randomized controlled trials and comprehensively assesses functional and psychological outcomes of DHIs in older patients with hip fractures, distinct from prior studies focusing solely on functional outcomes. While the 95% CI supports the potential of DHIs to improve hip function and functional independence, the wide 95% PI indicating substantial real-world response variability, which calls for cautious interpretation, informs the design of targeted DHI-based rehabilitation regimens, warranting further research into optimal techniques and dosages in clinical practice. Trial Registration: PROSPERO CRD42024626186; https://www.crd.york.ac.uk/PROSPERO/view/CRD42024626186

Associations Between Short-Video Platform Use and Health Across Health Distribution and Usage Behaviors in China: Cross-Sectional Questionnaire Study

Background: Short-video platforms, characterized by algorithmic curation and passive consumption, have emerged as dominant components of digital life. However, the associations between short-video platform use and health across different groups and usage behaviors remain understudied. Objective: This study investigates associations between short-video platform use and health, examining whether these relationships vary across health status, usage behaviors, and socioeconomic status. Methods: A cross-sectional study was conducted using multistage stratified sampling across eastern, central, and western China from July to September 2024. The inclusion criteria were age 18 years or older, ability to communicate effectively, and no cognitive disorders or mental disturbance. Of 7725 participants enrolled, 46.96% (n=3628) were male, and the average age was 65.49 (SD 8.39) years. The data were collected via face-to-face interviews using a structured questionnaire. Self-rated health and relative health deprivation (Kakwani index) were used to measure health. Quantile regression explored associations between whether using short-video platform and health varies across the health distribution, while linear regression examined associations of years, frequency, daily duration, and purpose diversity of short-video platform use with health. Moderating effect analysis explored the role of socioeconomic status in the relationship between the daily duration of use and health. Results: Coefficients were tested using 2-tailed tests, and statistical significance was defined as a 2-sided value less than .05. Quantile regression revealed heterogeneous associations. Compared to nonusers, short-video platform users had better self-rated health at the 70th to 90th quantiles and lower relative health deprivation at the 10th to 30th quantiles. However, the users at the 10th quantile of self-rated health had worse self-rated health (=−2.224, 95% CI −3.835 to −0.613). Longer engagement (≥3 y) correlated with lower relative health deprivation (=1.970, 95% CI 0.308-3.632), while daily use of 1‐4 hours was associated with poorer self-rated health (=−3.385, 95% CI −4.872 to −1.898; =−3.038, 95% CI −5.054 to −1.022) and higher relative health deprivation (=0.035, 95% CI 0.021-0.050;

Multi-omics analysis of BTF3L4 as a prognostic and immune biomarker in hepatocellular carcinoma

Transl Cancer Res. 2026 Feb 28;15(2):77. doi: 10.21037/tcr-2025-aw-2179. Epub 2026 Feb 11.

ABSTRACT

BACKGROUND: Hepatocellular carcinoma (HCC) exhibits notable characteristics, encompassing frequent recurrence, weak immunotherapeutic outcomes and unfavorable prognosis. BTF3L4 has been identified as a critical factor in the progression of various malignancies. However, its specific role in HCC remains to be elucidated. This investigation sought to examine BTF3L4 levels in HCC and BTF3L4's connection with clinical prognosis and immune infiltration.

METHODS: We performed an extensive multi-omics evaluation in the course of our research. Bioinformatics tools were utilized to assess BTF3L4 messenger RNA (mRNA) expression in HCC. Multiplex immunohistochemistry (mIHC) was utilized to examine BTF3L4 protein expression and to explore its correlation with tumor-infiltrating immune cells (TIICs). Cox regression analysis and Kaplan-Meier survival curves were applied to determine BTF3L4's impact on patient outcomes.

RESULTS: Our analysis revealed markedly elevated levels of both BTF3L4 mRNA and protein in HCC tissues. BTF3L4 protein abundance emerged as an independent predictor of reduced survival in patients with HCC. Furthermore, elevated BTF3L4 protein expression was positively associated with cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) expression and markedly negatively correlated with CD4+ T cells and CD66b+ neutrophils in HCC tissues.

CONCLUSIONS: This evidence indicates that BTF3L4 functions as a predictive indicator and is a potential candidate for HCC immunotherapy.

PMID:41815168 | PMC:PMC12971597 | DOI:10.21037/tcr-2025-aw-2179

Integrated multi-omics analysis reveals that MARCKS reprograms the immunosuppressive microenvironment to drive hepatocellular carcinoma progression

NPJ Precis Oncol. 2026 Mar 11. doi: 10.1038/s41698-026-01372-7. Online ahead of print.

ABSTRACT

Hepatocellular carcinoma (HCC) is one of the most lethal malignancies worldwide, and its progression is closely linked to the establishment of an immunosuppressive tumor microenvironment. Myristoylated alanine-rich C kinase substrate (MARCKS) has been implicated in tumor biology; however, its role in regulating immune interactions in HCC remains poorly defined. Here, we performed an integrated multi-omics analysis combining bulk transcriptomics, single-cell RNA sequencing, and spatial transcriptomics to systematically investigate the expression pattern and functional relevance of MARCKS in HCC. We found that MARCKS was significantly upregulated in HCC tissues and that high MARCKS expression was associated with aggressive clinicopathological features and unfavorable prognosis. Single-cell and spatial analyses revealed that MARCKS expression was enriched in myeloid cell populations within the tumor microenvironment. Functional annotation and mIF(Multiple immunofluorescence) validation demonstrated that MARCKS expression was associated with enhanced JAK/STAT3 signaling and M2-like macrophage polarization. Consistently, MARCKS silencing in HCC cell lines reduced STAT3 phosphorylation, suppressed malignant phenotypes in vitro, inhibited tumor growth in vivo, and diminished the capacity of tumor-derived conditioned media to promote macrophage M2 polarization. Together, these findings identify MARCKS as a key regulator of the immunosuppressive tumor microenvironment in HCC and highlight its potential as a therapeutic target for overcoming immune evasion.

PMID:41813922 | DOI:10.1038/s41698-026-01372-7

CSF1R T567M mutation induces microglial dysfunction and synaptic impairment in patient iPSC-derived cerebral organoids of CSF1R-related disorder

Cell Death Discovery, Published online: 12 March 2026; doi:10.1038/s41420-026-02995-2

CSF1R T567M mutation induces microglial dysfunction and synaptic impairment in patient iPSC-derived cerebral organoids of CSF1R-related disorder

Harnessing pyroptosis in breast cancer therapy: immunological mechanisms and emerging biomaterial strategies

Cell Death Discovery, Published online: 12 March 2026; doi:10.1038/s41420-026-02996-1

Harnessing pyroptosis in breast cancer therapy: immunological mechanisms and emerging biomaterial strategies

Biomolecular profiling for noninvasive health monitoring

Nature Biotechnology, Published online: 12 March 2026; doi:10.1038/s41587-026-03050-2

Kim et al. present mass spectrometry and wearable sensor technology as complementary approaches that, when integrated, can co-evolve to open new possibilities for noninvasive health monitoring.
  • ✇MIT Technology Review
  • Hustlers are cashing in on China’s OpenClaw AI craze Caiwei Chen
    Feng Qingyang had always hoped to launch his own company, but he never thought this would be how—or that the day would come this fast.  Feng, a 27-year-old software engineer based in Beijing, started tinkering with OpenClaw, a popular new open-source AI tool that can take over a device and autonomously complete tasks for a user, in January. He was immediately hooked, and before long he was helping other curious tech workers with less technical proficiency install the AI agent. Feng soon re
     

Hustlers are cashing in on China’s OpenClaw AI craze

11 March 2026 at 20:46

Feng Qingyang had always hoped to launch his own company, but he never thought this would be how—or that the day would come this fast. 

Feng, a 27-year-old software engineer based in Beijing, started tinkering with OpenClaw, a popular new open-source AI tool that can take over a device and autonomously complete tasks for a user, in January. He was immediately hooked, and before long he was helping other curious tech workers with less technical proficiency install the AI agent.

Feng soon realized this could be a lucrative opportunity. By the end of January, he had set up a page on Xianyu, a secondhand shopping site, advertising “OpenClaw installation support.” “No need to know coding or complex terms. Fully remote,” reads the posting. “Anyone can quickly own an AI assistant, available within 30 minutes.” 

At the same time, the broader Chinese public was beginning to catch on—and the tool, which had begun as a niche interest among tech workers, started to evolve into a popular sensation.

Feng quickly became inundated with requests, and he started chatting with customers and managing orders late into the night. At the end of February, he quit his job. His side gig has now grown into a full-fledged professional operation with over 100 employees. So far, the store has handled 7,000 orders, each worth about 248 RMB or approximately $34. 

“Opportunities are always fleeting,” says Feng. “As programmers, we are the first to feel the winds shift.”

Feng is among a small cohort of savvy early adopters turning China’s OpenClaw craze into cash. As users with little technical background want in, a cottage industry of people offering installation services and preconfigured hardware has sprung up to meet them. The sudden rise of these tinkerers and impromptu consultants shows just how eager the general public in China is to adopt cutting-edge AI—even when there are huge security risks. 

A “lobster craze”

“Have you raised a lobster yet?” 

Xie Manrui, a 36-year-old software engineer in Shenzhen, says he has heard this question nonstop over the past month. “Lobster” is the nickname Chinese users have given to OpenClaw—a reference to its logo.

Xie, like Feng, has been experimenting with OpenClaw since January. He’s built new open-source tools on top of the ecosystem, including one that visualizes the agent’s progress as an animated little desktop worker and another that lets users voice-chat with it. 

“I’ve met so many new people through ‘lobster raising,’” says Xie. “Many are lawyers or doctors, with little technical background, but all dedicated to learning new things.”

Lobsters are indeed popping up everywhere in China right now—on and offline. In February, for instance, the entrepreneur and tech influencer Fu Sheng hosted a livestream showing off OpenClaw’s capabilities that got 20,000 views. And just last weekend, Xie attended three different OpenClaw events in Shenzhen, each drawing more than 500 people. These self-organized, unofficial gatherings feature power users, influencers, and sometimes venture capitalists as speakers. The biggest event Xie attended, on March 7, drew more than 1,000 people; in the packed venue, he says, people were shoulder to shoulder, with many attendees unable to even get a seat.

Now China’s AI giants are starting to piggyback on the trend too, promoting their models, APIs,  and cloud services (which can be used with OpenClaw), as well as their own OpenClaw-like agents. Earlier this month, Tencent held a public event offering free installation support for OpenClaw, drawing long lines of people waiting for help, including elderly users and children.

This sudden burst in popularity has even prompted local governments to get involved. Earlier this month the government of Longgang, a district in Shenzhen, released several policies to support OpenClaw-related ventures, including free computing credits and cash rewards for standout projects. Other cities, including Wuxi, have begun rolling out similar measures.

These policies only catalyze what’s already in the air. “It was not until my father, who is 77, asked me to help install a ‘lobster’ for him that I realized this thing is truly viral,” says Henry Li, a software engineer based in Beijing. 

A programmer gold rush

What’s making this moment particularly lucrative for people with technical skills, like Feng, is that so many people want OpenClaw, but not nearly as many have the capabilities to access it. Setting it up requires a level of technical knowledge most people do not possess, from typing commands into a black terminal window to navigating unfamiliar developer platforms. On the hardware side, an older or budget laptop may struggle to run it smoothly. And if the tool is not installed on a device separate from someone’s everyday computer, or if the data accessible to OpenClaw is not properly partitioned, the user’s privacy could be at risk—opening the door to data leaks and even malicious attacks. 

Chris Zhao, known as “Qi Shifu” online, organizes OpenClaw social media groups and events in Beijing. On apps like Rednote and Jike, Zhao routinely shares his thoughts on AI, and he asks other interested users to leave their WeChat ID so he can invite them to a semi-private group chat. The proof required to join is a screenshot that shows your “lobster” up and running. Zhao says that even in group chats for experienced users, hardware and cloud setup remain a constant topic of discussion.

The relatively high bar for setting up OpenClaw has generated a sense of exclusivity, creating a natural opening for a service industry to start unfolding around it. On Chinese e-commerce platforms like Taobao and JD, a simple search for “OpenClaw” now returns hundreds of listings, most of them installation guides and technical support packages aimed at nontechnical users, priced anywhere from 100 to 700 RMB (approximately $15 to $100). At the higher end, many vendors offer to come to help you in person. 

Like Feng, most providers of these services are early adopters with some technical ability who are looking for a side gig. But as demand has surged, some have found themselves overwhelmed. Xie, the developer in Shenzhen who created tools to layer on OpenClaw, was asked by a friend who runs one such business to help out over the weekend; the friend had a customer who worked in e-commerce and had little technical experience, so Xie had to show up in person to get it done. He walked away with 600 RMB ($87) for the afternoon.

The growing demand has also pushed vendors like Feng to expand quickly. He has now standardized his operation into tiers: a basic installation, a custom package where users can make specific requests like configuring a preferred chat app, and an ongoing tutoring service for those who want a hand to hold as they find their footing with the technology.

Other vendors in China are making money combining OpenClaw with hardware. Li Gong, a Shenzhen-based seller of refurbished Mac computers, was among the first online sellers to do this—offering Mac minis and MacBooks with OpenClaw preinstalled. Because OpenClaw is designed to operate with deep access to a hard drive and can run continuously in the background unattended, many users prefer to install it on a separate device rather than on the one they use every day. This would help prevent bad actors from infiltrating the program and immediately gaining access to a wide swathe of someone’s personal information. Many turn to secondhand or refurbished options to keep the cost down. Li says that in the last two weeks, orders have increased eightfold.

Though OpenClaw itself is a new technology, the general practice of buying software bundles, downloading third-party packages, and seeking out modified devices is nothing new for many Chinese internet users, says Tianyu Fang, a PhD candidate studying the history of technology at Harvard University. Many users pay for one-off IT support services for tasks from installing Adobe software to jailbreaking a Kindle.

Still, not everyone is getting swept up. Jiang Yunhui, a tech worker based in Ningbo, worries that ordinary users who struggle with setup may not be the right audience for a technology that is still effectively in testing. 

“The hype in first-tier cities can be a little overblown,” he says. “The agent is still a proof of concept, and I doubt it would be of any life-changing use to the average person for now.” He argues that using it safely and getting anything meaningful out of it requires a level of technical fluency and independent judgment that most new users simply don’t have yet.

He’s not alone in his concerns. On March 10, the Chinese cybersecurity regulator CNCERT issued a warning about the security and data risks tied to OpenClaw, saying it heightens users’ exposure to data breaches.

Despite the potential pitfalls, though, China’s enthusiasm for OpenClaw doesn’t seem to be slowing.

Feng, now flush with the earnings from his operation, wants to use the momentum—and the capital—to keep building out his own venture with AI tools at the center of it.

“With OpenClaw and other AI agents, I want to see if I can run a one-person company,” he says. “I’m giving myself one year.”

Profiling of the mycobiome and metabolome: a comparative study of benign pulmonary nodules and lung adenocarcinoma

Front Cell Infect Microbiol. 2026 Feb 23;16:1732958. doi: 10.3389/fcimb.2026.1732958. eCollection 2026.

ABSTRACT

INTRODUCTION: Lung adenocarcinoma (LUAD), the most common subtype of non-small cell lung cancer, is a form of malignant pulmonary nodule that requires clinical differentiation from benign pulmonary nodules (BPN). The mechanisms underlying the development of LUAD are complex, and effective non-invasive methods for differentiating BPN from LUAD are lacking. This study aimed not only to distinguish BPN from LUAD using gut fungi and serum metabolites, but also to establish an integrated network of gut fungi-metabolite-cytokine interactions.

METHODS: Fecal and serum samples from individuals with BPN and patients with LUAD were subjected to internal transcribed spacer sequencing, ultra-performance liquid chromatography-tandem mass spectrometry, and multiplex Luminex assays to quantify gut fungi, metabolites, and cytokines, respectively.

RESULTS: A significant difference in gut fungal communities was observed between the BPN and LUAD groups. Multiple genera and species were more abundant in LUAD than in BPN. Docosapentaenoic acid n-6 (DPAn-6), indole-3-propionic acid (IPA), and interferon-γ-induced protein 10 (IP-10) were significantly elevated in the LUAD group. The integrated model established using a combination of gut fungi and metabolites demonstrated excellent performance in distinguishing BPN from LUAD. A network of interactions was established among differentially abundant gut fungi, serum metabolites, and cytokines.

CONCLUSION: Our study identifies a novel panel of fungal and metabolite biomarkers for differentiating between BPN and LUAD, and constructs a multi-omics network that provides new insights into investigating the mechanistic role of gut mycobiota dysbiosis in LUAD.

PMID:41809995 | PMC:PMC12968269 | DOI:10.3389/fcimb.2026.1732958

Regulatory mechanisms of ALKBH5/CIITA axis in the synergistic modulation of hepatocellular carcinoma radiotherapy and immunotherapy

Genes Immun. 2026 Mar 10. doi: 10.1038/s41435-026-00382-6. Online ahead of print.

ABSTRACT

The prognosis for hepatocellular carcinoma remains grim. Combining radiotherapy with immune checkpoint blockade (ICB) has shown potential to enhance therapeutic outcomes, yet there is a pressing need for further advancements. Our previous research demonstrated that this combined approach suppresses ALKBH5 gene expression and increases m6A modification levels in hepatocellular carcinoma tissues. High-throughput sequencing and detailed molecular analysis revealed that inhibiting ALKBH5 amplifies CIITA m6A modifications post-therapy. This modulation triggers MHC II molecule expression in tumors, facilitating the presentation of tumor-associated antigens to CD4 + T lymphocytes and the recruitment of CD8 + T cells for an anti-tumor immune response. Building on these findings, we engineered a CIITA vector with a specific site mutation to confirm that the regulation of CIITA by the combined radiotherapy and immunotherapy is mediated through m6A methylation. Consequently, we established a comprehensive network involving ALKBH5, CIITA, MHC II, and CD4+ and CD8 + T cells. To elucidate the role and underlying molecular mechanisms of this combined therapy in reshaping the tumor immune microenvironment for hepatocellular carcinoma, we employed multi-omics approaches across in vitro, animal model, and clinical multi-dimensional studies, offering novel insights for enhancing treatment efficacy.

PMID:41807814 | DOI:10.1038/s41435-026-00382-6

Multimodal electron microscopy of halide perovskite interfacial dynamics

Nature, Published online: 11 March 2026; doi:10.1038/s41586-026-10238-8

A multimodal in situ electron microscopy approach enables direct visualization of structural and chemical evolution in a working halide perovskite light-emitting diode with nanometre precision.

Risk-adaptive therapy guided by dynamic ctDNA in nasopharyngeal carcinoma

Nature, Published online: 11 March 2026; doi:10.1038/s41586-026-10244-w

A clinical trial testing whether monitoring ctDNA clearance during treatment for nasopharyngeal cancer could be used to inform decisions about an individual’s subsequent therapeutic programme shows promising results.
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