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Received β€” 26 March 2026 ⏭

Research on the compatibility mechanism of the Tingli Dazao Xiefei Decoction by multi-organ metabolomics strategy

βœ‡(Multiomics OR Omics) AND (Lung OR gastric OR Hepatocellular)
By: Rui-Feng Wang Β· Pei-Pei Yuan Β· Long Chen Β· Zhen-Kai Zhang Β· Bing-Xian Zhang Β· Lin Chen Β· Shu-Miao Yuan Β· Yan-Fang Zhao Β· Ning Zhou Β· Xiao-Ke Zheng Β· Wei-Sheng Feng
23 March 2026 at 18:00

J Ethnopharmacol. 2026 Mar 21:121548. doi: 10.1016/j.jep.2026.121548. Online ahead of print.

ABSTRACT

ETHNOPHARMACOLOGICAL RELEVANCE: The Tingli Dazao Xiefei Decoction (TD) is a traditional phlegm-eliminating prescription composed of Descurainia sophia (L.) Webb. ex Prantl (TLZ) and Ziziphus jujuba Mill. (DZ), which can relieve lung, heart and kidney injury in asthma. TLZ acts as the monarch drug in the TD. Based on the research mode of "material basis of traditional Chinese medicinal properties can be divided and combined", we have confirmed that the flavonoid glycosides components /the oligosaccharide components/the fatty oil component (FG/Oli/FO) are effective components of TLZ. However, the compatibility mechanism of the TD, and the contribution of the effective components of TLZ to the efficacy were still unclear.

AIM OF THE STUDY: To clarify the compatibility mechanism of TD, and the contribution of the effective components of TLZ to the efficacy from a comprehensive perspective of lung, heart, and kidney.

METHODS: First, we chose the asthma model corresponding to the efficacy of TD in purging the lungs and relieving asthma, and the rats were divided into the normal (NC) group, model (M) group, dexamethasone (DEX) group, and treatment groups of TD/TLZ/DZ/FO+DZ/Oli+DZ/FG+DZ. Second, metabolomics and network pharmacology were applied to elucidate the comprehensive protective effect of TD/FG+DZ/Oli+DZ/FO+DZ. Third, the multi-omics results were validated using Western blotting, RT-qPCR, flow cytometry, and immunofluorescence.

RESULTS: FO+DZ/Oli+DZ/FG+DZ had different degrees of protective effects against lung/heart/kidney injury in asthma. In metabolomics research, the principal component analysis (PCA) and cluster analysis results showed that the TLZ group was closer to TD group than DZ group, the FO+DZ and Oli+DZ group clustered with TD/NC groups in the lung and kidney, and the FO+DZ and FG+DZ group clustered with TD/NC groups in the heart. Pathway enrichment analysis suggested that the comprehensive protective effect of TLZ and its effective components combined with DZ on lung/heart/kidney may be achieved by regulating the arginine and proline metabolism, alanine, aspartate and glutamate metabolism, and unsaturated fatty acid biosynthesis. Multi-organ metabolomics and network pharmacology revealed consistent biological functions in KEGG pathways. Validation experiment showed that TLZ and its effective components combined with DZ could reverse the abnormal expression of proteins and RNA related to inflammation, airway remodeling, excitotoxicity, and energy-supply, apoptosis at different levels. Furthermore, FO+DZ may reduce asthma damage by inhibiting the FABP4/PPAR-Ξ³/NF-ΞΊB signaling pathway.

CONCLUSION: TLZ played the key role in TD, and FO had the best therapeutic effect on each organ; the efficacy of Oli was mainly reflected in reducing lung and kidney damage, and FG was mainly involved in enhancing energy metabolism in the heart. These findings proved that traditional Chinese medicine could exert comprehensive efficacy in a 'multi-components trigger multi-channel' way.

PMID:41871629 | DOI:10.1016/j.jep.2026.121548

Research on the compatibility mechanism of the Tingli Dazao Xiefei Decoction by multi-organ metabolomics strategy

βœ‡Omics In Lung
By: Rui-Feng Wang Β· Pei-Pei Yuan Β· Long Chen Β· Zhen-Kai Zhang Β· Bing-Xian Zhang Β· Lin Chen Β· Shu-Miao Yuan Β· Yan-Fang Zhao Β· Ning Zhou Β· Xiao-Ke Zheng Β· Wei-Sheng Feng
23 March 2026 at 18:00

J Ethnopharmacol. 2026 Mar 21:121548. doi: 10.1016/j.jep.2026.121548. Online ahead of print.

ABSTRACT

ETHNOPHARMACOLOGICAL RELEVANCE: The Tingli Dazao Xiefei Decoction (TD) is a traditional phlegm-eliminating prescription composed of Descurainia sophia (L.) Webb. ex Prantl (TLZ) and Ziziphus jujuba Mill. (DZ), which can relieve lung, heart and kidney injury in asthma. TLZ acts as the monarch drug in the TD. Based on the research mode of "material basis of traditional Chinese medicinal properties can be divided and combined", we have confirmed that the flavonoid glycosides components /the oligosaccharide components/the fatty oil component (FG/Oli/FO) are effective components of TLZ. However, the compatibility mechanism of the TD, and the contribution of the effective components of TLZ to the efficacy were still unclear.

AIM OF THE STUDY: To clarify the compatibility mechanism of TD, and the contribution of the effective components of TLZ to the efficacy from a comprehensive perspective of lung, heart, and kidney.

METHODS: First, we chose the asthma model corresponding to the efficacy of TD in purging the lungs and relieving asthma, and the rats were divided into the normal (NC) group, model (M) group, dexamethasone (DEX) group, and treatment groups of TD/TLZ/DZ/FO+DZ/Oli+DZ/FG+DZ. Second, metabolomics and network pharmacology were applied to elucidate the comprehensive protective effect of TD/FG+DZ/Oli+DZ/FO+DZ. Third, the multi-omics results were validated using Western blotting, RT-qPCR, flow cytometry, and immunofluorescence.

RESULTS: FO+DZ/Oli+DZ/FG+DZ had different degrees of protective effects against lung/heart/kidney injury in asthma. In metabolomics research, the principal component analysis (PCA) and cluster analysis results showed that the TLZ group was closer to TD group than DZ group, the FO+DZ and Oli+DZ group clustered with TD/NC groups in the lung and kidney, and the FO+DZ and FG+DZ group clustered with TD/NC groups in the heart. Pathway enrichment analysis suggested that the comprehensive protective effect of TLZ and its effective components combined with DZ on lung/heart/kidney may be achieved by regulating the arginine and proline metabolism, alanine, aspartate and glutamate metabolism, and unsaturated fatty acid biosynthesis. Multi-organ metabolomics and network pharmacology revealed consistent biological functions in KEGG pathways. Validation experiment showed that TLZ and its effective components combined with DZ could reverse the abnormal expression of proteins and RNA related to inflammation, airway remodeling, excitotoxicity, and energy-supply, apoptosis at different levels. Furthermore, FO+DZ may reduce asthma damage by inhibiting the FABP4/PPAR-Ξ³/NF-ΞΊB signaling pathway.

CONCLUSION: TLZ played the key role in TD, and FO had the best therapeutic effect on each organ; the efficacy of Oli was mainly reflected in reducing lung and kidney damage, and FG was mainly involved in enhancing energy metabolism in the heart. These findings proved that traditional Chinese medicine could exert comprehensive efficacy in a 'multi-components trigger multi-channel' way.

PMID:41871629 | DOI:10.1016/j.jep.2026.121548

Received β€” 25 February 2026 ⏭

WorldGUI: An Interactive Benchmark for Desktop GUI Automation from Any Starting Point

βœ‡cs.AI, q-bio.NC updates on arXiv.org
By: Henry Hengyuan Zhao Β· Kaiming Yang Β· Wendi Yu Β· Difei Gao Β· Mike Zheng Shou
24 February 2026 at 13:00
arXiv:2502.08047v4 Announce Type: replace Abstract: Recent progress in GUI agents has substantially improved visual grounding, yet robust planning remains challenging, particularly when the environment deviates from a canonical initial state. In real applications, users often invoke assistance mid-workflow, where software may be partially configured, steps may have been executed in different orders, or the interface may differ from its default setup. Such task-state variability is pervasive but insufficiently evaluated in existing GUI benchmarks. To address this gap, we introduce WorldGUI, a benchmark covering ten widely used desktop and web applications with tasks instantiated under diverse, systematically constructed initial states. These variations capture realistic human-computer interaction settings and enable diagnostic evaluation of an agent's ability to recover, adapt plans, and handle non-default contexts. We further present WorldGUI-Agent, a simple and model-agnostic framework that organizes planning and execution around three critique stages, improving reliability in dynamic environments. Experiments demonstrate that state-of-the-art GUI agents exhibit substantial performance degradation under non-default initial conditions, revealing limited robustness and fragile planning behaviors. Our benchmark and framework provide a foundation for developing more adaptable and reliable GUI agents. The code and data are available at https://github.com/showlab/WorldGUI.

VideoMind: A Chain-of-LoRA Agent for Temporal-Grounded Video Reasoning

βœ‡cs.AI, q-bio.NC updates on arXiv.org
By: Ye Liu Β· Kevin Qinghong Lin Β· Chang Wen Chen Β· Mike Zheng Shou
24 February 2026 at 13:00
arXiv:2503.13444v3 Announce Type: replace-cross Abstract: Videos, with their unique temporal dimension, demand precise grounded understanding, where answers are directly linked to visual, interpretable evidence. Despite significant breakthroughs in text-based reasoning with large language models, multi-modal reasoning - especially for videos - remains limited. In this work, we fill this gap by introducing VideoMind, a novel video-language agent for temporal-grounded video reasoning. Our method involves two key innovations: (1) We identify four essential capabilities for grounded video reasoning and propose a role-based agentic workflow, comprising a planner to coordinate roles, a grounder for temporal event localization, a verifier to assess event candidates, and an answerer for question answering. (2) To efficiently integrate these roles during inference, we propose a novel Chain-of-LoRA mechanism, where a unified base model with multiple LoRA adapters is leveraged to enable seamless role switching, balancing efficiency and flexibility. Extensive experiments on 15 benchmarks across Grounded VideoQA, Video Temporal Grounding, and General VideoQA tasks demonstrate the effectiveness of the proposed scheme in advancing video agent, test-time scaling, and long-form video reasoning. Code, models, datasets, and demos are available at https://videomind.github.io/.
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