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Dynamic Targetable Extracellular Vesicle Surface Proteins Monitor Depth of Response to CAR T Therapy

Res Sq [Preprint]. 2026 Mar 18:rs.3.rs-8913641. doi: 10.21203/rs.3.rs-8913641/v1.

ABSTRACT

Extracellular vesicles (EVs) represent a promising liquid biopsy platform in multiple myeloma (MM). We developed an MM EV Surface Protein Assay to quantify and dynamically monitor four MM EV subpopulations defined by targetable MM surface proteins (BCMA, CD38, GPRC5D, and CD319) across 336 serial blood samples from 45 relapsed/refractory MM (RRMM) patients treated with anti-BCMA chimeric antigen receptor (CAR) T-cell therapy. All four MM EV subpopulations significantly decreased in 43 patients with initial response, while BCMA+, GPRC5D+, and CD319+ MM EVs increased in 19 patients with progression, and antigen escape was detected by BCMA+ MM EVs. MM EV subpopulations differentiated minimal residual disease (MRD) status and complemented MRD for detecting early relapse before clinical progression. Notably, CD319+ MM EVs were early predictors of progression-free and overall survival in MRD-negative patients. This assay enables noninvasive monitoring of deep response, progression, and antigen escape, and stratifies survival in MRD-negative patients with RRMM.

PMID:41890853 | PMC:PMC13015583 | DOI:10.21203/rs.3.rs-8913641/v1

XAI and Few-shot-based Hybrid Classification Model for Plant Leaf Disease Prognosis

arXiv:2603.06676v1 Announce Type: cross Abstract: Performing a timely and accurate identification of crop diseases is vital to maintain agricultural productivity and food security. The current work presents a hybrid few-shot learning model that integrates Explainable Artificial Intelligence (XAI) and Few-Shot Learning (FSL) to address the challenge of identifying and classifying the stages of disease of the diseases of maize, rice, and wheat leaves under limited annotated data conditions. The proposed model integrates Siamese and Prototypical Networks within an episodic training paradigm to effectively learn discriminative disease features from a few examples. To ensure model transparency and trustworthiness, Gradient-weighted Class Activation Mapping (Grad-CAM) is employed for visualizing key decision regions in the leaf images, offering interpretable insights into the classification process. Experimental evaluations on custom few-shot datasets developed in the study prove that the model consistently achieves high accuracy, precision, recall, and F1-scores, frequently exceeding 92% across various disease stages. Comparative analyses against baseline FSL models further confirm the superior performance and explainability of the proposed approach. The framework offers a promising solution for real-world, data-constrained agricultural disease monitoring applications.
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