A vasoactive intestinal peptide (VIP)-based protein carrier self-assembles with respiratory syncytial virus circular RNA vaccines for intranasal delivery, inducing systemic antibodies, mucosal IgA, and Th1-biased protection in mice. This platform offers a protein-guided strategy for respiratory mucosal RNA vaccination and broadens the application of VIP in vaccine delivery.

Tissue patterning events of early human embryo development, including an embryo-disc with primitive streak-like structures, are captured using an in vitro model where embryonic stem cells are cultured with extra-embryonic cell types.