Nature Biotechnology, Published online: 31 March 2026; doi:10.1038/s41587-026-03083-7

Single-molecule 4Pi microscopy is simplified and made accessible by using a single objective.

Histidine scanning represents a broadly applicable technique for the identification of critical interaction sites within TCRs and other mechanosensory receptors to enhance receptor signaling strength and augment therapeutic efficacy via the catch bond mechanism.