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A pervasive RT–qPCR artifact inflates RNA knockdown by RNA-targeting CRISPR

Nature Biotechnology - Issue - nature.com science feeds

By: Leslie Watkins · Alan Zhu · Bin Wu

1 September 2026 at 08:00

Nature Biotechnology, Published online: 01 September 2026; doi:10.1038/s41587-026-03291-1

A quantification artifact confounds CRISPR-mediated RNA knockdown.