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Scalable homology detection with ERAST
Nature Biotechnology, Published online: 01 April 2026; doi:10.1038/s41587-026-03051-1
ERAST speeds up homology search and provides a vector database for 1 billion biological sequences.
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Scalable single-cell total RNA sequencing unifies coding and noncoding transcriptomics
Nature Biotechnology, Published online: 31 March 2026; doi:10.1038/s41587-026-03068-6
Simultaneous profiling of adenylated and non-adenylated RNAs reveals regulatory programs across diverse cell types.
Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging
Nature Biotechnology, Published online: 31 March 2026; doi:10.1038/s41587-026-03083-7
Single-molecule 4Pi microscopy is simplified and made accessible by using a single objective.
Massively parallel promoter mapping identifies edits to boost sorghum gene expression
Nature Biotechnology, Published online: 30 March 2026; doi:10.1038/s41587-026-03060-0
Non-transgenic crop improvement via cis-regulatory editing is limited by rare activating alleles and incomplete promoter maps. A massively parallel reporter assay in sorghum protoplasts quantifies more than 30,000 CRISPR-mimicking variants across three photosynthesis genes, finely mapping cis-regulatory modules and pinpointing compact edits that drive upregulated protein production.
Engineering macrophage-targeted trispecific immune cell engagers for tumor immunotherapy
Nature Biotechnology, Published online: 30 March 2026; doi:10.1038/s41587-026-03104-5
Immune cell engagers (ICEs) have emerged as a promising strategy for cancer immunotherapy, but their efficacy in solid tumors remains limited, partly as a result of the difficulty of coordinating activating and inhibitory signals within the same immune cell. Here, we report a logic-gated cis-targeting strategy that enables ICEs to coordinate activating and inhibitory signals within macrophages, thereby enhancing tumor clearance in mouse models of solid cancers.
Unpaired data as a first-order challenge in single-cell and spatial proteomics
Nature Biotechnology, Published online: 27 March 2026; doi:10.1038/s41587-026-03074-8
Unpaired data as a first-order challenge in single-cell and spatial proteomics
Mapping <i>cis</i>-regulatory mutations at scale in sorghum enables modulation of gene expression
Nature Biotechnology, Published online: 27 March 2026; doi:10.1038/s41587-026-03046-y
Variant mapping of sorghum promoters identifies CRISPR-accessible mutations that upregulate gene expression.
High-resolution metagenome assembly for modern long reads with myloasm
Nature Biotechnology, Published online: 27 March 2026; doi:10.1038/s41587-026-03053-z
A long-read metagenome assembly method recovers circular and complete genomes better than existing tools.
Generalist biological artificial intelligence in modeling the language of life
Nature Biotechnology, Published online: 20 March 2026; doi:10.1038/s41587-026-03064-w
This Review discusses the promises and pitfalls of biological AI algorithms and presents a vision for generalist biological artificial intelligence, in which models can perform diverse tasks across biological domains.
Functional integration of an autologous engineered esophagus in a large-animal model
Nature Biotechnology, Published online: 20 March 2026; doi:10.1038/s41587-026-03043-1
A tissue-engineered esophageal segment is functional in minipigs.
An engineered <i>E. coli</i> strain sustains intratumoral nitric oxide production to boost effectiveness of tumor immunotherapy
Nature Biotechnology, Published online: 18 March 2026; doi:10.1038/s41587-026-03055-x
An engineered Escherichia coli Nissle 1917 strain carrying a synthetic arginine–nitric oxide (NO) circuit enables sustained intratumoral NO production that promotes vascular normalization and immune reprogramming. This reshaped tumor microenvironment synergizes with blockade of the immune checkpoint PD-L1 to reinvigorate CD8+ T cells and drive durable tumor regression in mouse models.
Sustained nitric oxide production by engineered <i>E. coli</i> remodels the tumor microenvironment and potentiates immunotherapy
Nature Biotechnology, Published online: 18 March 2026; doi:10.1038/s41587-026-03054-y
Solid tumors are sensitized to anti‑PD‑L1 immunotherapy by engineered E. coli to produce nitric oxide.
Single-molecule peptide sequencing through reverse translation of peptides into DNA
Nature Biotechnology, Published online: 18 March 2026; doi:10.1038/s41587-026-03061-z
Peptides are sequenced by converting each amino acid into amplifiable DNA barcodes.
Precise, minimally evolved adenine base editors generated through mutation reversion analysis
Nature Biotechnology, Published online: 18 March 2026; doi:10.1038/s41587-026-03045-z
Base editor precision is increased by reverting established mutations.
Balenciaga debuts eight-legged yarns
Nature Biotechnology, Published online: 17 March 2026; doi:10.1038/s41587-026-03062-y
Balenciaga debuts eight-legged yarns
FDA backpedals to review Moderna mRNA flu vaccine
Nature Biotechnology, Published online: 17 March 2026; doi:10.1038/s41587-026-03067-7
FDA backpedals to review Moderna mRNA flu vaccine
A logic-gated trispecific engager enhances macrophage killing of cancer cells in solid tumors
Nature Biotechnology, Published online: 13 March 2026; doi:10.1038/s41587-026-03057-9
A trispecific macrophage engager amplifies the antitumor response of macrophages in solid tumors.
Biomolecular profiling for noninvasive health monitoring
Nature Biotechnology, Published online: 12 March 2026; doi:10.1038/s41587-026-03050-2
Kim et al. present mass spectrometry and wearable sensor technology as complementary approaches that, when integrated, can co-evolve to open new possibilities for noninvasive health monitoring.
Quantifying endosomal escape in vivo to guide lipid nanoparticle design
Nature Biotechnology, Published online: 11 March 2026; doi:10.1038/s41587-026-03047-x
A lysosomal barcoding strategy to quantify endosomal escape of nucleic acids in vivo assesses the performance of branched ionizable lipids for potent liver delivery.
In vivo endosomal escape assay identifies mechanisms for efficient hepatic LNP delivery
Nature Biotechnology, Published online: 11 March 2026; doi:10.1038/s41587-026-03022-6
Endosomal escape of lipid nanoparticles is quantified and optimized in vivo.