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Site-specific DNA insertion into the human genome with engineered recombinases

Nature Biotechnology - Issue - nature.com science feeds

By: Alison Fanton · Liam J. Bartie · Juliana Q. Martins · Vincent Q. Tran · Laine Goudy · Courtney Kernick · Matthew G. Durrant · Jingyi Wei · Zev Armour-Garb · April Pawluk · Silvana Konermann · Alexander Marson · Luke A. Gilbert · Theodore L. Roth · Patrick D. Hsu

6 November 2025 at 08:00

Nature Biotechnology, Published online: 06 November 2025; doi:10.1038/s41587-025-02895-3

Engineered DNA recombinases efficiently and specifically insert genetic cargos without the use of landing pads.

Bridge RNAs direct programmable recombination of target and donor DNA

Nature - Issue - nature.com science feeds

By: Matthew G. Durrant · Nicholas T. Perry · James J. Pai · Aditya R. Jangid · Januka S. Athukoralage · Masahiro Hiraizumi · John P. McSpedon · April Pawluk · Hiroshi Nishimasu · Silvana Konermann · Patrick D. Hsu

26 June 2024 at 08:00

Nature, Published online: 26 June 2024; doi:10.1038/s41586-024-07552-4

A bispecific non-coding RNA expressed by the IS110 family of mobile genetic elements forms the basis of a programmable genome-editing system that enables the insertion, excision or inversion of specific target DNA sequences.