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PRXL2B facilitates the progression of hepatocellular carcinoma and the therapeutic efficacy of oncolytic adenovirus H101 through the PI3K/AKT/PD-L1 axis

Biosci Trends. 2026 May 21. doi: 10.5582/bst.2026.01000. Online ahead of print.

ABSTRACT

Oncolytic adenovirus H101 has shown antitumor activity in hepatocellular carcinoma (HCC), but the molecular determinants of treatment response remain unclear. In this study, a Hepa1-6 subcutaneous tumor model was established in C57BL/6 mice and treated with intratumoral H101, followed by integrated transcriptomic and proteomic analyses to identify candidate genes associated with H101 response. PRXL2B was selected for further investigation using public multi-omics datasets, tissue microarray-based immunohistochemistry, in vitro functional assays, mechanistic analyses, and in vivo validation experiments. Integrated multi-omics analyses identified PRXL2B as a candidate gene downregulated after H101 treatment. Public datasets and tissue-based validation further showed that PRXL2B was upregulated in HCC tissues. In MHCC97H and HCCLM3 cells, PRXL2B knockdown inhibited proliferation, migration, and invasion, promoted apoptosis and cell-cycle arrest, and enhanced the antitumor effect of H101. Mechanistically, PRXL2B silencing reduced AKT phosphorylation and PD-L1 expression. In vivo, PRXL2B knockdown suppressed tumor growth, and the combination of PRXL2B knockdown and H101 produced the strongest antitumor effect. These findings indicate that PRXL2B promotes malignant phenotypes in HCC and may modulate H101 efficacy through the PI3K/AKT/PD-L1 axis. Targeting PRXL2B may therefore represent a potential strategy to enhance the therapeutic efficacy of oncolytic virus therapy in HCC.

PMID:42161529 | DOI:10.5582/bst.2026.01000

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MuDD: A Multimodal Deception Detection Dataset and GSR-Guided Progressive Distillation for Non-Contact Deception Detection

arXiv:2603.26064v2 Announce Type: replace-cross Abstract: Non-contact automatic deception detection remains challenging because visual and auditory deception cues often lack stable cross-subject patterns. In contrast, galvanic skin response (GSR) provides more reliable physiological cues and has been widely used in contact-based deception detection. In this work, we leverage stable deception-related knowledge in GSR to guide representation learning in non-contact modalities through cross-modal knowledge distillation. A key obstacle, however, is the lack of a suitable dataset for this setting. To address this, we introduce MuDD, a large-scale Multimodal Deception Detection dataset containing recordings from 130 participants over 690 minutes. In addition to video, audio, and GSR, MuDD also provides Photoplethysmography, heart rate, and personality traits, supporting broader scientific studies of deception. Based on this dataset, we propose GSR-guided Progressive Distillation (GPD), a cross-modal distillation framework for mitigating the negative transfer caused by the large modality mismatch between GSR and non-contact signals. The core innovation of GPD is the integration of progressive feature-level and digit-level distillation with dynamic routing, which allows the model to adaptively determine how teacher knowledge should be transferred during training, leading to more stable cross-modal knowledge transfer. Extensive experiments and visualizations show that GPD outperforms existing methods and achieves state-of-the-art performance on both deception detection and concealed-digit identification.
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HoloTrauma 3X Triadic AI Co reasoning for robot assisted emergency maxillofacial reconstruction

npj Digital Medicine, Published online: 04 April 2026; doi:10.1038/s41746-026-02573-x

HoloTrauma 3X Triadic AI Co reasoning for robot assisted emergency maxillofacial reconstruction
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