By fusing MCM5, a subunit of the eukaryotic MCM2–7 helicase complex, to the N terminus of spCas9 (MCCas), the MCCas fusion protein enhances large-size fragment knockin via homologous recombination, reduces insertions and deletions (indels), and enables efficient large-size fragment insertions in human cells and rabbit embryos.

Oncogene, Published online: 31 August 2026; doi:10.1038/s41388-026-03974-8

Frequently amplified SHANK2 promotes esophageal squamous cell carcinoma progression through the DVL/YAP axis