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Systems pharmacology approaches decipher the anti-cancer efficacy of ethnopharmacological agents in hepatocellular carcinoma
Sci Rep. 2025 Dec 17;15(1):43996. doi: 10.1038/s41598-025-27744-w.
ABSTRACT
Hepatocellular carcinoma (HCC) poses a significant global health burden with limited therapeutic efficacy. Chinese herbal medicines (CHMs) offer multi-target potential, yet their systematic screening and mechanistic elucidation remain challenging. We established a high-throughput multi-omics platform integrating transcriptomics, proteomics, and deep learning (autoencoder and multiple kernel learning) to screen 187 medicinal plants. Five CHMs candidates were identified and shown to modulate hub genes (e.g., AKR1B10, HMGCR, THBS1) and key pathways (TNF/IL-17/MAPK, apoptosis, ferroptosis). Proteomic validation and functional assays confirmed their roles in suppressing proliferation, migration, and inducing apoptosis in HCC cells. This study provides a robust, data-driven pipeline for natural anti-HCC drug discovery, linking specific hub genes to CHM efficacy and offering novel insights into precision ethnopharmacology.
PMID:41408124 | DOI:10.1038/s41598-025-27744-w
Leveraging LLMs for Structured Data Extraction from Unstructured Patient Records
ValuePilot: A Two-Phase Framework for Value-Driven Decision-Making
Enhancing Transparency and Traceability in Healthcare AI: The AI Product Passport
Graph AI generates neurological hypotheses validated in molecular, organoid, and clinical systems
Complex Mathematical Expression Recognition: Benchmark, Large-Scale Dataset and Strong Baseline
TF-MCL: Time-frequency Fusion and Multi-domain Cross-Loss for Self-supervised Depression Detection
Why Text Prevails: Vision May Undermine Multimodal Medical Decision Making
Assessing High-Risk Systems: An EU AI Act Verification Framework
A data-physics hybrid generative model for patient-specific post-stroke motor rehabilitation using wearable sensor data
A Multicenter Benchmark of Multiple Instance Learning Models for Lymphoma Subtyping from HE-stained Whole Slide Images
COMMA: A Communicative Multimodal Multi-Agent Benchmark
A Knowledge Graph-based Retrieval-Augmented Generation Framework for Algorithm Selection in the Facility Layout Problem
Virology’s most wanted: the influenza virus
Nature, Published online: 17 December 2025; doi:10.1038/d41586-025-03607-2
The death toll and economic damage associated with flu highlight its role as one of the most harmful viruses in history.Two-stage prompting framework with predefined verification steps for evaluating diagnostic reasoning tasks on two datasets
npj Digital Medicine, Published online: 16 December 2025; doi:10.1038/s41746-025-02146-4
Two-stage prompting framework with predefined verification steps for evaluating diagnostic reasoning tasks on two datasetsSingle-cell and spatial transcriptomic characterization of pulmonary pleomorphic carcinoma
Commun Biol. 2025 Dec 16;8(1):1773. doi: 10.1038/s42003-025-09162-w.
ABSTRACT
Pulmonary pleomorphic carcinoma (PPC) is a rare subtype of lung cancer that comprises both epithelial and sarcomatoid components. The molecular basis of PPC, including the cellular dynamics of its components, remains largely unknown. To elucidate potential therapeutic targets for PPC, we perform a multi-omics analysis incorporating digital spatial profiling and single-cell RNA sequencing (scRNA-seq). PPC exhibits diverse driver gene alterations, including MET exon 14 skipping mutation (METex14) and ALK fusion. In spatial transcriptomics, MET gene and protein are overexpressed exclusively within the epithelial component and not in the sarcomatoid component, even in patients harboring METex14. Epithelial-mesenchymal transition (EMT)-related transcriptional changes, along with extracellular matrix (ECM) remodeling between the epithelial and sarcomatoid components, are observed. scRNA-seq identifies cell populations within the epithelial component that contribute to the malignant transformation and differentiation of the sarcomatoid component. They are characterized by an intermediate EMT state with ECM remodeling signature, suggesting their potential as novel therapeutic targets for PPC.
PMID:41402584 | PMC:PMC12708732 | DOI:10.1038/s42003-025-09162-w